Vaccinia Virus and Poxvirology

副标题: Methods and Protocols (Methods in Molecular Biology)

ISBN: 9781588292292

作者: Stuart Neal Isaacs

出版社: Humana Press

出版年: 2004-04-19

页数: 416

定价: USD 104.00

装帧: Hardcover

内容简介

A comprehensive collection of cutting-edge methods to study and work with the vaccinia virus and other poxvirus gene transcriptions. These readily reproducible techniques can be used for the construction and characterization of recombinant viruses; for the study of poxvirus gene transcription and DNA replication; for the investigation of the binding, entry, and movement of the virus in host cells; and for the use of the virus in immunologic and cell-cell fusion assays. Other methods cover poxvirus bioinformatics, aspects of viral pathogenesis at both a protein and an animal model level, and the study of immune responses to poxviruses-the latter a critical ability given the important role of vaccinia virus in smallpox vaccination and their potential role as vaccine vectors directed against infectious agents and cancer.