Basic Cloning Procedures

ISBN: 9783540635390

出版社: Springer Verlag

页数: 169

定价: $ 99.38

装帧: Pap

内容简介

This manual introduces the reader to basic methods used in the isolation, cloning and analysis of genetic material. The protocols include RT-PCR amplification, gene cloning, hybridization analysis and sequencing of nucleic acids, PCR-based site-specific mutagenesis, analysis of protein DNA-specific interaction, cell-free protein synthesis and product electrophoretic and immunological analysis. Each protocol includes short background information, a detailed description of the necessary materials, step-by-step procedures, a troubleshooting guide and useful practical hints.