RNA Interference in Practice

副标题: Principles, Basics, and Methods for Gene Silencing in C.elegans, Drosophila, and Mammals

ISBN: 9783527310203

出版社: Wiley-VCH

出版年: 2005-1-31

页数: 336

定价: USD 180.00

装帧: Hardcover

内容简介

This hands-on guide to RNA interference brings the power of targeted gene silencing to any laboratory with the basic equipment for handling nucleic acids. In easy-to-follow, step-by-step protocols you will learn: How RNAi works in worms, flies and mammals How to design the most efficient RNAi constructs How to achieve transient, stable and conditional RNAi in cell cultures How to determine the efficiency of an RNAi experiment and how to use RNAi for gene therapy All the protocols have been thoroughly tested in the author's own laboratory, and she provides examples of successful experiments and troubleshooting hints to help in establishing your own successful RNAi experiments. Also includes a list of suppliers for RNAi reagents and equipment as well as a glossary of terms.