Protein Purification

ISBN: 9780471746614

出版社: John Wiley & Sons Inc

出版年: 2011-3

页数: 548

定价: 954.00元

装帧: HRD

内容简介

This thoroughly revised and updated third edition provides an overview of the principles and methodology of the most frequently used biochemical separation techniques for proteins used today. Balancing theory, procedures, and applications, it offers professionals and students in biochemistry, organic chemistry, and analytical chemistry quick access to a wide range of important techniques, including: Gel filtration and ion exchange chromatography Affinity and covalent chromatography Reversed phase and hydrophobic interaction chromatography Ligands from combinatorial libraries Electrophoresis in gels Immunoelectrophoresis and capillary electrophoresis Two-dimensional electrophoresis in proteomics Protein elution and blotting techniques Large-scale electrophoresis processes